anti-proliferative and apoptotic effects of ziziphus jujube on cervical and breast cancer cells

Authors

mohammad reza abedini department of pharmacology, faculty of medicine, birjand university of medical sciences, birjand, iran

nafiseh erfanian department of biology, payam-e noor university of esfahan, esfahan, iran

habibollah nazem department of biology, payam-e noor university of esfahan, esfahan, iran

sara jamali cellular & molecular research center, birjand university of medical sciences, birjand, iran

abstract

objectives: ziziphus jujube (jujube) plant has exhibited numerous medicinal and pharmacological properties including antioxidant and anti-inflammatory effects. this study was carried out to investigate its anti-cancer and pro-apoptotic abilities in human cervical and breast cancer cells in vitro. materials and methods: the cervical ov2008 and breast mcf-7 cancer cells were incubated with different concentrations of jujube aqueous extraction (0-3 mg/ml) for various times (0-72 h). cell viability was assessed by trypan blue and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay. the expression of two apoptosis-related genes in treated cells evaluated by quantitative real time -pcr analysis. results: jujube significantly inhibited cancer cell viability in a dose- and time- dependent manner. herb-induced apoptosis was associated with enhanced expression of bax and decreased bcl2 gene leading eventually to a time-dependent six fold increase in the bax/bcl-2 ratio. conclusions: these results indicated that jujube may be a natural potential and promising agent to prevent or treat human cancers.

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Journal title:
avicenna journal of phytomedicine

جلد ۶، شماره ۲، صفحات ۱۴۲-۱۴۸

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